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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
29/09/2006 |
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Data da última atualização: |
20/09/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
NOGUEIRA, R. T.; QUEIROZ, S. C. do N. de; IMAMURA, P. M. |
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Afiliação: |
R. T. NOGUEIRA, IQ/Unicamp; SONIA CLAUDIA DO N DE QUEIROZ, CNPMA; P. M. IMAMURA, IQ/Unicamp. |
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Título: |
Semi-preparative HPLC separation of terpenoids from the seed pods of Hymenaea courbaril var. stilbocarpa. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Journal of Liquid Chromatography & Related Technologies, New York, v. 25, p 59-67, 2002. |
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Idioma: |
Inglês |
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Conteúdo: |
Column chromatography of the ethyl acetate extract of the seed pod resin of Hymenaea courbaril var. stilbocarpa furnished a fraction containing a mixture of two new clerodanes and a known clerodane diterpene. Due to the similarity of the structures, they could not be separated by ordinary chromatography even using silica gel impregnated with AgNO 3. Thus, an intense study was made in order to optimize the semipreparative separation of this mixture by HPLC (high performance liquid chromatograph) using several different stationary and mobile phases. The best conditions for separation of these diterpenes was on octadecyl-bonded silica with methanol:water:formic acid (85:15:1, v/v/v) as mobile phase, flow rate at 2 mL/min, and UV detection at 240 nm. The fractions obtained were analyzed by gas chromatography-mass spectrometry (GC-MS). |
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Thesagro: |
Cromatografia; Hymenaea Courbaril. |
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Categoria do assunto: |
W Química e Física |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/163838/1/Nogueira-HPLC-6384.pdf
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Marc: |
LEADER 01420naa a2200169 a 4500
001 1015040
005 2017-09-20
008 2002 bl uuuu u00u1 u #d
100 1 $aNOGUEIRA, R. T.
245 $aSemi-preparative HPLC separation of terpenoids from the seed pods of Hymenaea courbaril var. stilbocarpa.$h[electronic resource]
260 $c2002
520 $aColumn chromatography of the ethyl acetate extract of the seed pod resin of Hymenaea courbaril var. stilbocarpa furnished a fraction containing a mixture of two new clerodanes and a known clerodane diterpene. Due to the similarity of the structures, they could not be separated by ordinary chromatography even using silica gel impregnated with AgNO 3. Thus, an intense study was made in order to optimize the semipreparative separation of this mixture by HPLC (high performance liquid chromatograph) using several different stationary and mobile phases. The best conditions for separation of these diterpenes was on octadecyl-bonded silica with methanol:water:formic acid (85:15:1, v/v/v) as mobile phase, flow rate at 2 mL/min, and UV detection at 240 nm. The fractions obtained were analyzed by gas chromatography-mass spectrometry (GC-MS).
650 $aCromatografia
650 $aHymenaea Courbaril
700 1 $aQUEIROZ, S. C. do N. de
700 1 $aIMAMURA, P. M.
773 $tJournal of Liquid Chromatography & Related Technologies, New York$gv. 25, p 59-67, 2002.
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Embrapa Meio Ambiente (CNPMA) |
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| 1. |  | LORANDI, S.; GIRARDON, J. C.; MAIER, J. C.; AVILA, V. S. de. Efficiency of use of alternative foods to inducing molt. In: WORLD´S POULTRY CONGRESS, 24., 2012, Salvador. Abstract... Salvador: WSPA, 2012. 1 CD-ROM. World's Poultry Science Journal, v. 68, supl. 1, 2012. Projeto/Plano de Ação: 11.11.11.111.| Tipo: Artigo em Anais de Congresso |
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